A Switch Protein Adapter for Anti-LILRB4 CAR-T Cells.

• Tác giả: Zhiqiang An, Heyu Chen, Ryan Huang, Samuel John, Qi Lou, Lingxiao Tan, Jingjing Xie, Cheng Cheng Zhang, Ningyan Zhang
• Ngôn ngữ: eng
• Thông tin xuất bản: Germany : European journal of immunology , 2025
• Bộ sưu tập: NCBI
• ID: 89788

Tóm tắt

Chimeric antigen receptor-T cell (CAR-T) immunotherapy has shown remarkable results for the treatment of certain hematologic malignancies. A redirection strategy that utilizes clinically relevant CAR-T cells in combination with adapter proteins may be an effective strategy to target other hematologic and solid cancers. We established a fusion antibody-based strategy with flexibility to target multiple tumor types in combination with a novel anti-leukocyte immunoglobulin-like receptor-B 4 (LILRB4) CAR-T cell. Specifically, we engineered switch protein (SwP) adapters containing the LILRB4 extracellular domain fused to either an anti-CD19 or anti-CD20 single-chain variable fragment (scFv). These SwPs were sufficient to stimulate anti-LILRB4 CAR-T cells against SwP-tagged LILRB4