Recent theory and research suggest that elevated pain tolerance may be an important predictor of nonsuicidal self-injury (NSSI) engagement, particularly among individuals high in emotional dysregulation. However, researchers have yet to examine the role of state pain tolerance in NSSI behaviours in daily life, or the moderating effects of emotional dysregulation. In the present study, the link between pain tolerance and NSSI, as well as the moderating effects of emotional dysregulation, were examined using a daily diary approach. Participants included 130 emerging adults (Mage = 17.96, 72 % female) with recent NSSI engagement who completed a baseline survey and 14 days of daily diary assessments. Participants reported on their daily pain tolerance and NSSI (i.e., urges, engagement, frequency, and NSSI versatility). Using hierarchical linear modelling, it was found that state pain tolerance predicted same day NSSI engagement, NSSI frequency, and NSSI versatility, particularly for those with high trait emotion dysregulation. For individuals high in emotional dysregulation, pain tolerance also predicted next-day NSSI versatility. Pain tolerance may be relevant in NSSI risk assessment and prevention.