CAR-T cell targeting three receptors on autoreactive B cells for systemic lupus erythematosus therapy.

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Tác giả: Daniel Feinberg, Hunter Gibbons, Branden S Moriarity, Reshmi Parameswaran, Marissa Troje, Vinayak Uppin, Beau R Webber

Ngôn ngữ: eng

Ký hiệu phân loại: 004.33 Real-time processing

Thông tin xuất bản: England : Journal of autoimmunity , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 90574

Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by dysregulated B cell activation, autoantibody production, and nephritis. B cell activating factor (BAFF) overexpression enhances autoreactive B-cell survival, driving autoimmunity. BAFF specific belimumab and CD20 specific rituximab antibodies are used for SLE therapy but are not curative, highlighting the need for alternative B cell depletion therapies. Here, we use BAFF ligand based chimeric antigen receptor T (CAR-T) cells targeting BAFFr, BCMA and TACI expressed on mature B cells and plasma cells. BAFF CAR-T cells efficiently killed B cells after co-culture with peripheral blood mononuclear cells (PBMCs) from SLE patients and in a patient derived SLE xenograft humanized mouse model developed by injecting patient PBMCs into immunocompromised mice. We also generated murine CD8
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