Kinetic and structural investigation of the 4-allyl syringol oxidase from Streptomyces cavernae.

• Tác giả: Laura Alvigini, Daniel Eggerichs, Andrea Mattevi, Tobias Rapsch, Dirk Tischler, Heiner G Weddeling, Nils Weindorf
• Ngôn ngữ: eng
• Ký hiệu phân loại: 709.04073 Historical, geographic, persons treatment of fine and decorative arts
• Thông tin xuất bản: United States : Archives of biochemistry and biophysics , 2025
• Bộ sưu tập: NCBI
• ID: 90847

Tóm tắt

4-Phenol oxidases are proposed to be involved in the utilization of lignin-derived aromatic compounds. While enzymes with selectivity towards 4-hydroxyphenyl and guaiacyl motifs are well described, we identified the first syringyl-specific oxidase from Streptomyces cavernae (Sc4ASO) only very recently. Here, in-depth studies were conducted to unravel the molecular origins of the outstanding selectivity of Sc4ASO. Kinetic experiments revealed high activities on dimethoxylated substrates (up to 2.9 ± 0.1 s