Activation of megakaryocytic leukemia 1 in endothelial cells contributes to diabetic retinopathy in mice.

• Tác giả: Yuhua Ding, Xiaofen Feng, Fei Wang, Yuwen Zhu
• Ngôn ngữ: eng
• Ký hiệu phân loại: 636.0885 Animal husbandry
• Thông tin xuất bản: Netherlands : Life sciences , 2025
• Bộ sưu tập: NCBI
• ID: 90972

Tóm tắt

AIMS: Diabetic retinopathy (DR) represents one of the most devastating sequences in patients with diabetes. Endothelial dysfunction is a key pathological feature of and contributing factor to DR. In the present study we investigated the role of megakaryocytic leukemia 1 (MKL1) in DR pathogenesis. METHODS AND MATERIALS: DR was induced in mice by feeding with a high-fat diet (HFD). The Mkl1-Rosa26-KI mice were crossed to the Cdh5-Cre KEY FINDINGS: In cultured human primary retinal endothelial cells exposure to high glucose promoted nuclear translocation of MKL1 without altering its mRNA or protein expression. MKL1 knockdown ameliorated whereas MKL1 over-expression exacerbated high glucose induced impairment of endothelial barrier function. Compared to wild type littermates, MKL1 SIGNIFICANCE: Our data demonstrate that MKL1 may contribute to diabetic retinopathy by regulating endothelial behavior. Targeting MKL1 with small-molecule inhibitors can be considered as a therapeutic solution for the treatment of diabetic retinopathy.