Aptamers, discovered in the 1990s, have marked a significant milestone in the fields of therapeutics and diagnostics. This review provides a comprehensive survey of aptamers, focusing on their diagnostic applications. It especially encapsulates a decade of aptamer, encompassing research, patents, and market trends. The unique properties and inherent stability of aptamers are discussed, highlighting their potential for various clinical applications. It goes on to introduce biosensor design, emphasizing the advantages of aptamers over antibodies as conventional molecular recognition interface. The operation and design of aptasensors are examined, with a focus on single- and dual-site binding configurations and their respective recognition modes. Paper-based sensors are highlighted as cost-effective, user-friendly alternatives that are gaining widespread adoption, particularly in point-of-care platforms.