Zebrafish Leg1a and Leg1b are two homologous proteins sharing high sequence homology. Previous studies have revealed that leg1a and leg1b genes are important for early liver development and Leg1a and Leg1b are liver-produced serum proteins, however, whether they play a physiological role in the zebrafish liver remains unknown. Here, we carry out an analysis of the bulk RNA sequencing (RNA-seq) data and find that lipid metabolic pathways are the most prominently affected biological processes in the leg1a