The FOREVER YOUNG FLOWER (FYF/AGL42) gene plays an important role in regulating the flower development especially the flowering time, and senescence and abscission of floral organs. Melon is an important horticultural crop, and the flower development has an important influence on pollination, fertilization and final fruit yield. However, the function of FYF homolog is still unknown in melon. In this study, the characteristic of melon CmFYF was analyzed combining with molecular biology, developmental biology and biochemical tools. CmFYF was present in all detected tissues of melon, but its expression level was significantly higher in shoot apex of lateral branches and male flowers than that in other tissues. Subcellular localization revealed that CmFYF was located in the nucleus. CmFYF was able to respond to multiple hormone and environmental signals including GA