Clonal lineage tracing and transcriptomics of cortical progenitor populations reveal maintenance of differentiation potential.

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Tác giả: Shawar Ali, Daniela Lozano Casasbuenas, Sara Ciganovic, Danyon Harkins, Stephanie Ouzikov, Teodora Tockovska, Samuel Watanabe, Scott A Yuzwa

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: United States : Stem cell reports , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 96746

Postnatal neocortical development is a complex period wherein radial glial progenitors (RGPs) complete excitatory neurogenesis and transition to the production of glia. Here, we take advantage of a multi-layered lineage tracing tool pbacBarcode, to examine the contributions of individual cortical RGPs to the postnatal cortex. We reveal that some individual cortical RGPs are multipotent and give rise to olfactory bulb interneurons, astrocytes, and oligodendrocytes in a ∼2:1:1 ratio. We provide evidence that differentiation potential into terminal cell types is maintained as late as post-natal day (P)4, suggesting that a population decline model, as opposed to cell fate restriction, underlies postnatal neocortical development. Moreover, a pool of proliferative intermediary cells, which may represent a multipotent postnatal intermediate progenitor cell population, may contribute to the production of the three major cell types. Lastly, we examine RGP postnatal contribution to oligodendrocytes and show that oligodendrocyte progenitor founder cell production by cortical RGPs is largely complete by P3.
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