Flavonoids represent an important research topic in the analytical chemistry of secondary plant metabolites. During habitual laboratory determinations, preliminary quantitative analysis is often associated with in vitro colorimetric assessment. Total flavonoid content (TFC) is used as screening method with high relevance in the chemical analysis of plants and derived products, being typically applied before HPLC-MS phytochemical profiling. Its importance stems from affordability, simplicity, rapidity, and low cost. The AlCl