Refining Ligand Poses in RNA/Ligand Complexes of Pharmaceutical Relevance: A Perspective by QM/MM Simulations and NMR Measurements.

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Tác giả: Paolo Carloni, Gia Linh Hoang, Sybille Krauss, Thomas Magauer, Davide Mandelli, Manuel Röck, Giulia Rossetti, Jörg B Schulz, Aldo Tancredi, Martin Tollinger

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: United States : The journal of physical chemistry letters , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 9721

Predicting the binding poses of ligands targeting RNAs is challenging. Here, we propose that using first-principles quantum mechanics/molecular mechanics (QM/MM) simulations, which incorporate automatically polarization effects, can help refine the structural determinants of ligand/RNA complexes in aqueous solution. In fact, recent advances in massively parallel computer architectures (such as exascale machines), combined with the power of machine learning, are greatly expanding the domain of applicability of these types of notoriously expensive simulations. We corroborate this proposal by carrying out a QM/MM-based study on a ligand targeting CAG repeat-RNA, involved in Huntington's disease. The calculations indeed show a clear improvement in the ligand binding properties, and they are consistent with the NMR measurements, also performed here. Thus, this type of approach may be useful for practical applications in the design of ligands targeting RNA in the near future.
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