Nail tissue, as a keratinized biomatrix, serves as a valuable indicator of chronic human exposure to toxic pollutants. The analysis of nail samples has emerged as a prominent method for human exposure assessment, primarily due to its non-invasive sampling procedure and ease of sample storage and transportation. This review examines recent applications of nail analysis in human biomonitoring of toxic organic compounds and heavy metals, along with the various pretreatment methods that have been developed. Studies of human nail samples have revealed distinctive patterns in toxic pollutant accumulation. Brominated flame retardants exhibit significant occupational exposure differences, with concentrations reaching 2.20 × 10