The practical applications of essential oils (EOs) are limited by their hydrophobicity, volatility and instability. To overcome these drawbacks, the incorporation of EOs into porous materials is recommended. Chitosan (CS)-based composite cryogels as hosts for thymol as a volatile model terpenoid were developed using a double cross-linking of CS with glutaraldehyde (GA) and monochlorotriazinyl-β-cyclodextrin (MCT-β-CD), either in one-pot synthesis or through sequential cross-linking, first with GA and then with MCT-β-CD, resulting in CSCAV composites. Cross-linking with GA occurred under cryogelation conditions, while cross-linking with MCT-β-CD took place by heating at 60-80 °C. The composite cryogels were characterized by FTIR,