With the rapid growth of multi-level health needs, precise and real-time health sensing systems have become increasingly pivotal in personal health management and disease prevention. Natural polysaccharides demonstrate immense potential in healthcare sensors by leveraging their superior biocompatibility, biodegradability, environmental sustainability, as well as diverse structural designs and surface functionalities. This review begins by introducing a variety of natural polysaccharides, including cellulose, alginates, chitosan, hyaluronic acid, and starch, and analyzing their structural and functional distinctions, which offer extensive possibilities for sensor design and construction. Further, we summarize several principal sensing mechanisms, such as piezoresistivity, piezoelectricity, capacitance, triboelectricity, and hygroelectricity, which provide a theoretical and technological foundation for developing high-performance healthcare sensing devices. Additionally, the review discusses the most recent applications of natural polysaccharide-based sensors in diverse healthcare contexts, including human body motion tracking, respiratory and heartbeat monitoring, electrophysiological signal recording, body temperature variation detection, and biomarker analysis. Finally, prospective development directions are proposed, such as the integration of artificial intelligence for real-time data analysis, the design of multifunctional devices that combine sensing with therapeutic functionalities, and advancements in remote monitoring and smart wearable technologies. This review aims to provide valuable insights into the development of next-generation healthcare sensors and propose novel research directions for personalized medicine and remote health management.