Extensive gene mining and facile engineering of a novel carbonyl reductase for asymmetric synthesis of anti-aging (S)-Pro-Xylane from d-xylose.

• Tác giả: Yuan-Qiang Cai, Zhe Dou, Pei-Ru Hu, Lin-Lin Song, Hao-Nan Tang, Ya-Jun Wang, Yi-Cheng Wang, Zhen-Qi Weng
• Ngôn ngữ: eng
• Thông tin xuất bản: Netherlands : International journal of biological macromolecules , 2025
• Bộ sưu tập: NCBI
• ID: 97654

Tóm tắt

Carbonyl reductases (CRs) are promising biological macromolecules for the asymmetric synthesis of chiral secondary alcohols. A novel CR was evolved for efficient conversion of d-xylose into anti-aging (S)-Pro-Xylane. Through extensive gene mining, an active carbonyl reductase ZmCR was identified from Zygofabospora marxiana with high reductive activity towards d-xylose-derived 1-C-(β-d-xylopyranosyl)-acetone (XPSA) and in situ cofactor regeneration capability. Using a three-round of concise engineering strategy, a hextuple mutant C85V/V128C/S129A/V161S/Y166H/T217A (M6) was identified from merely 157 mutants with specific activity of 46.00 U·mg