Staphylococcal enterotoxins have long been studied due to the food-borne illness caused and complex intracellular virulence regulation involved. Along with the popularized application of cold chain production of meat products, there is important significance to explore the influence of low temperature on enterotoxin genes expression correlated to regulators under meat cultivation. In this study, SEA and SEB type Staphylococcus aureus strains (designated Sa08 and Sa13 respectively) were cultivated on chicken meat at 8 and 25 °C, typical virulence regulators (agrA, saeS, srrB, rot, codY and icaA), enterotoxins (sea and seb) and metabolic factors (pyk and pyruvate, purR and xanthosine monophosphate) were monitored. As a result, cell density remained stable (10