The aim of this study was to ascertain the current status of healthcare workers' (HCWs) willingness to receive the influenza vaccination and to identify the influencing factors in Quzhou. A self-administered questionnaire was used to investigate the cognition and vaccination intention of in-service HCWs in 10 medical and health institutions in Quzhou from July to September in 2022, and the influencing factors of vaccination intention were analyzed by Logistic regression. A total of 228 questionnaires were returned, with the effective rate of 95.2%. The influenza vaccination coverage rate of HCWs in 2021-2022 season was 22.6%, and the willingness rate in 2022-2023 season was 56.7%. Logistic regression showed that township medical institutions, junior professional titles, people who thought vaccines were effective and people who had been vaccinated with influenza vaccine in the past increased their willingness to get influenza vaccine. The influenza vaccination coverage rate of HCWs in Quzhou is low, and the vaccination willingness is affected by many factors. Providing free vaccines for HCWs and targeted education are the key measures to reduce the vaccine hesitancy for HCWs.