SNOM Imaging of a Crypt-Like Feature in Adenocarcinoma Associated with Barrett's Oesophagus [electronic resource]

• Tác giả: Fermi National Accelerator Laboratory, United States, United States. Dept. of Energy. Office of High Energy Physics
• Ngôn ngữ: eng
• Ký hiệu phân loại: 614.5 Incidence of and public measures to prevent specific diseases and kinds of diseases
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of High Energy Physics ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2018
• Mô tả vật lý: Size: Article No. 1700518 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 260355
• Chủ đề: 1. 60 applied life sciences; 2. Adenocarcinoma; 3. Dna; 4. Infrared radiation; 5. Scanning near-field optical microscopy; 6. Tissue

Tóm tắt

 The development of more accurate and sensitive diagnostic techniques is a key factor in efforts to improve cancer survival rates. The technique of infrared aperture fibre scanning near-field optical microscopy (IR-SNOM),together with radiation from the infrared free-electron laser (IR-FEL) on ALICEat Daresbury Laboratory (UK), has been used to obtain IR images of a crypt-like feature and the surrounding tissue
  the tissue was taken from a patient with oesophageal adenocarcinoma and with a history of Barrett?s oesophagus. We have shown that the DNA signal is enhanced relative to other contributions in the region of the crypt, and the glycoprotein signal shows a less pronounced increase in the region of the crypt. The Amide II signal is found to be anti-correlated with the DNA and glycoprotein profiles. The absorbance of the Amide II signal is found to differ for three different types of cancer tissue. In conclusion, high-resolution IR images of the crypt reveal additional structure that would not be resolved in diffraction-limited techniques.