This study provides framework for decision makers to more fully evaluate the toxicology and biodegradability of the tier three gasoline blendstocks developed under Co-Optima. These Co-Optima blendstocks were compared to gasoline surrogate compounds, providing guidance for future consideration as the project progresses. Due to the complexity of the subject area, all compounds were considered only in their pure form, not as complex mixtures with gasoline, which was outside the scope of this study. The metrics applied to the blendstocks were environmental transport and fate, environmental and human health toxicity, and environmental regulatory framework. The blendstocks (cyclopentanone
diisobutylene
2-methylfuran
2,5-dimethylfuran
isobutanol
ethanol
and isopropanol) presented limited human toxicity based on the information available. All the blendstocks migrated primarily into air or water, with little mobility between these compartments. Coupled with aerobic and/or anaerobic biodegradability, long term environmental persistence is unlikely, with the only exception being diisobutylene, which was not biodegradable. Additional work should focus on the environmental impact of these blendstocks in complex mixtures, such as gasoline, which will represent more real world scenarios.