This 2 volume-set of IFIP AICT 583 and 584 constitutes the refereed proceedings of the 16th IFIP WG 12.5 International Conference on Artificial Intelligence Applications and Innovations, AIAI 2020, held in Neos Marmaras, Greece, in June 2020.* The 70 full papers and 5 short papers presented were carefully reviewed and selected from 149 submissions. They cover a broad range of topics related to technical, legal, and ethical aspects of artificial intelligence systems and their applications and are organized in the following sections: Part I: classification
  clustering - unsupervised learning -analytics
  image processing
  learning algorithms
  neural network modeling
  object tracking - object detection systems
  ontologies - AI
  and sentiment analysis - recommender systems. Part II: AI ethics - law
  AI constraints
  deep learning - LSTM
  fuzzy algebra - fuzzy systems
  machine learning
  medical - health systems
  and natural language. *The conference was held virtually due to the COVID-19 pandemic.