Quantum yield (QY) is a fundamental parameter in luminescence study. This paper introduces a novel SAFE method for determining the fluorescence quantum yield of 5-(2-(dimethylamino)-6-methoxypyrimidin-4-yl)-furan-2-carbaldehyde, which was synthesized from pyrimidine derivatives through the Suzuki cross-coupling reaction. A key contribution of this study is integrating measuring tools into a single device, as opposed to using two separate devices typically found in commercial applications. The study utilizes a commercial fluorescence spectrometer capable of recording absorption and emission spectra simultaneously, ensuring precise and efficient measurements. The fluorescence quantum yields of anthracene, quinine sulfate, tyrosine, and the synthesized pharmacological compound were investigated. Quinine sulfate (QS), dissolved in 0.05 M sulfuric acid, served as the reference standard on a traceable fluorometer. Measurements were conducted using laboratory-designed quantum yield measurement software based on the SAFE method, yielding results with high accuracy relative to standard values. The quantum yields for tyrosine in water, anthracene, and 5-(2-(dimethylamino)-6-methoxypyrimidin-4-yl)-furan-2-carbaldehyde in ethanol were determined at specific excitation wavelengths. For the first set of excitation wavelengths (λ₁) at 270, 330, and 385 nm, the QY values were found to be 13.089, 28.51, and 27.126, respectively. In a second set of excitation wavelengths (λ₂) at 273, 335, and 397 nm, the corresponding QY values were determined to be 13.091, 29.153, and 27.354, with quinine sulfate serving as the reference standard at λ₁ (299 nm) and λ₂ (310 nm). These findings demonstrate the effectiveness of the SAFE method in accurately determining fluorescence quantum yields, offering significant utility for characterizing luminescent compounds and evaluating pharmacological substances.